The purpose of this study was to assess the effect of motivation on the job performance of employees, using the case study of Barclays Bank Ghana Limited. To effectively achieve the intended aim of this study, a self-administered questionnaire was developed to assess the various motivational schemes and policies that are used in Barclays Bank Ghana, the key drivers and factors that influence motivation in Barclays Bank Ghana, as well as the influence of motivation of employees on their job performance. The Statistical Package for the Social Sciences (SPSS) was used to analyze the data collected from 50 respondents comprising of 40 general employees and 10 management staff of Barclays Bank Ghana. Various descriptive statistics and regression analysis were performed to assess the objectives of the study. The main finding of the study indicates that motivation has a significant positive effect on performance and the study recommends that banks should combine both monetary and non-monetary motivation, strong financial projection tools and balanced labor regulation to improve employee job performance.