In Ghana, freight transport is growing continuously every year due to its location and business processes. However, road transport carries 86% of freight despite its numerous negative impacts. Hence, the government has invested in rail transport, with 70% of its capacity is for freight transport in her busy freight transport corridor (eastern transport regions of Ghana). Thus, awareness of criteria considered when deciding on freight transport becomes vital. Hence, this study aims to improve the understanding of the factors of freight transport mode selection in Ghana from the decision-making process by identifying criteria that affect their decisions on mode transportation. The combination of Fuzzy AHP and Topsis is used to find the weights and suggest suitable alternatives for the decision-makers in the Eastern transport regions of Ghana. The result of this study shows that the criteria to consider when selecting freight transport mode in the regions are prioritized in other of Transport cost (0.6544), transport Time factors (0.2562), reliability, and flexibility (0.0605), and security, Risk of damage and lose factors (0.0287). Additionally, the suitable mode(s) of transportation in the stated corridor is owned truck carrier transport compared to the railroad, road-barge, and Contracted Carrier, thus, in descending order. The results provide organizations to prioritize these factors when deciding to select freight transport mode. At the same time, the government must remove some inputs that result in high transport costs, enforce policies, and invest in the appropriate mode.